TY - JOUR
T1 - Characterization of topoisomerase II α and minichromosome maintenance protein 2 expression in anal carcinoma
AU - Scapulatempo-Neto, Cristovam
AU - Veo, Carlos
AU - Fregnani, José Humberto T.G.
AU - Lorenzi, Adriana
AU - Mafra, Allini
AU - Melani, Armando G.F.
AU - Loaiza, Edgar Antonio Alemán
AU - Rosa, Luciana Albina Reis
AU - DE Oliveira, Cristina Mendes
AU - Levi, José Eduardo
AU - Longatto-Filho, Adhemar
N1 - Publisher Copyright:
© 2017, Spandidos Publications. All rights reserved.
PY - 2017/3
Y1 - 2017/3
N2 - The present study aimed to ascertain the significance of topoisomerase II α (TOP2A) and minichromosome maintenance protein (MCM) 2 expression in anal carcinoma. A total of 75 anal lesions were retrieved from the files of the Department of Pathology of Barretos Cancer Hospital (Barretos, Brazil) in order to verify the human papillomavirus (HPV) statuses of these lesions and characterize the immunohistochemical expression levels of TOP2A and MCM2 in anal carcinoma, as these are important markers for cervical HPV-induced lesions; their expression was also compared with respect to p16 and Ki-67. The vast majority of the cases tested positive for HPV16 (84%); 1 case tested positive for both HPV16 and HPV18. Positive HPV16 status was more frequent in early stages than in advanced stages (P=0.008). Positive immunohistochemical reactivity for MCM2 and TOP2A protein was observed in 71.6 and 100% of cases, respectively. Positive reactivity for p16 was significantly associated (P=0.001) with histological grade, and was more commonly expressed in squamous cell carcinoma than adenocarcinomas. HPV16 was strongly associated with positive p16 protein expression (76.6%). However, the high expression of Ki-67 combined with the high expression of p16 was predominantly observed in Stage III-IV cases. MCM2, TOP2A, p16 and Ki-67 exhibited intense positive staining in the anal lesions, indicating that these markers were significantly and constantly expressed in anal carcinoma.
AB - The present study aimed to ascertain the significance of topoisomerase II α (TOP2A) and minichromosome maintenance protein (MCM) 2 expression in anal carcinoma. A total of 75 anal lesions were retrieved from the files of the Department of Pathology of Barretos Cancer Hospital (Barretos, Brazil) in order to verify the human papillomavirus (HPV) statuses of these lesions and characterize the immunohistochemical expression levels of TOP2A and MCM2 in anal carcinoma, as these are important markers for cervical HPV-induced lesions; their expression was also compared with respect to p16 and Ki-67. The vast majority of the cases tested positive for HPV16 (84%); 1 case tested positive for both HPV16 and HPV18. Positive HPV16 status was more frequent in early stages than in advanced stages (P=0.008). Positive immunohistochemical reactivity for MCM2 and TOP2A protein was observed in 71.6 and 100% of cases, respectively. Positive reactivity for p16 was significantly associated (P=0.001) with histological grade, and was more commonly expressed in squamous cell carcinoma than adenocarcinomas. HPV16 was strongly associated with positive p16 protein expression (76.6%). However, the high expression of Ki-67 combined with the high expression of p16 was predominantly observed in Stage III-IV cases. MCM2, TOP2A, p16 and Ki-67 exhibited intense positive staining in the anal lesions, indicating that these markers were significantly and constantly expressed in anal carcinoma.
KW - Anal carcinoma
KW - Human papillomavirus
KW - Ki-67
KW - Minichromosome maintenance protein 2
KW - Topoisomerase II α
KW - p16
UR - http://www.scopus.com/inward/record.url?scp=85011805105&partnerID=8YFLogxK
U2 - 10.3892/ol.2017.5650
DO - 10.3892/ol.2017.5650
M3 - Article
AN - SCOPUS:85011805105
SN - 1792-1074
VL - 13
SP - 1891
EP - 1898
JO - Oncology Letters
JF - Oncology Letters
IS - 3
ER -