TY - JOUR
T1 - Changes in lecithin
T2 - cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer
AU - Mihajlovic, Marija
AU - Gojkovic, Tamara
AU - Vladimirov, Sandra
AU - Miljkovic, Milica
AU - Stefanovic, Aleksandra
AU - Vekic, Jelena
AU - Zeljkovic, Dejan
AU - Trifunovic, Bratislav
AU - Kotur-Stevuljevic, Jelena
AU - Spasojevic-Kalimanovska, Vesna
AU - Zeljkovic, Aleksandra
N1 - Publisher Copyright:
© 2018 The Canadian Society of Clinical Chemists
PY - 2019/1
Y1 - 2019/1
N2 - Background: Previous studies revealed decreased level of high-density lipoprotein cholesterol (HDL–C) as important factor for development of colorectal cancer (CRC). Quantity and structure of HDL particles depend on activities of lipid transfer proteins lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP), but this topic is largely unexplored in CRC. The main objective of this study was to investigate activities of LCAT and CETP in patients with CRC. Additionally, we analyzed activity of paraoxonase-1 (PON-1), as a main carrier of HDL-antioxidant function. Materials and methods: Ninety-nine CRC patients and 101 healthy individuals were included. LCAT and CETP activities were assessed by measuring rates of formation and transfer of cholesteryl esters. PON-1 paraoxonase and arylesterase activities were measured. Results: Lower levels of HDL-C (p <.001) were observed in cohort of patients, alongside with decreased LCAT (p <.050) and increased CETP activity (p <.050). Both PON-1 activities were diminished in CRC (p <.050 and p <.001 respectively). Univariate logistic regression singled out HDL-C level (OR = 0.218, p <.001), CETP activity (OR = 1.010, p <.01) and mass (OR = 0.994, p <.001) as possible markers of elevated CRC risk. CETP mass maintained its predictive significance when adjusted for traditional risk factors and level of oxidative stress (OR = 0.993, p <.001; OR = 0.982, p <.050, respectively). Conclusion: Our results demonstrated increased CETP and decreased LCAT and PON-1 activities in CRC patients. In preliminary analysis CETP mass was identified as potential significant predictor of CRC development, suggesting that alterations in HDL-C levels, alongside with changes in HDL structure might have a role in carcinogenesis.
AB - Background: Previous studies revealed decreased level of high-density lipoprotein cholesterol (HDL–C) as important factor for development of colorectal cancer (CRC). Quantity and structure of HDL particles depend on activities of lipid transfer proteins lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP), but this topic is largely unexplored in CRC. The main objective of this study was to investigate activities of LCAT and CETP in patients with CRC. Additionally, we analyzed activity of paraoxonase-1 (PON-1), as a main carrier of HDL-antioxidant function. Materials and methods: Ninety-nine CRC patients and 101 healthy individuals were included. LCAT and CETP activities were assessed by measuring rates of formation and transfer of cholesteryl esters. PON-1 paraoxonase and arylesterase activities were measured. Results: Lower levels of HDL-C (p <.001) were observed in cohort of patients, alongside with decreased LCAT (p <.050) and increased CETP activity (p <.050). Both PON-1 activities were diminished in CRC (p <.050 and p <.001 respectively). Univariate logistic regression singled out HDL-C level (OR = 0.218, p <.001), CETP activity (OR = 1.010, p <.01) and mass (OR = 0.994, p <.001) as possible markers of elevated CRC risk. CETP mass maintained its predictive significance when adjusted for traditional risk factors and level of oxidative stress (OR = 0.993, p <.001; OR = 0.982, p <.050, respectively). Conclusion: Our results demonstrated increased CETP and decreased LCAT and PON-1 activities in CRC patients. In preliminary analysis CETP mass was identified as potential significant predictor of CRC development, suggesting that alterations in HDL-C levels, alongside with changes in HDL structure might have a role in carcinogenesis.
KW - Cholesteryl ester transfer protein
KW - Colorectal cancer
KW - Lecithin:cholesterol acyltransferase
KW - Paraoxonase-1
KW - Prooxidant/antioxidant balance
UR - http://www.scopus.com/inward/record.url?scp=85057453833&partnerID=8YFLogxK
U2 - 10.1016/j.clinbiochem.2018.11.010
DO - 10.1016/j.clinbiochem.2018.11.010
M3 - Article
C2 - 30500525
AN - SCOPUS:85057453833
SN - 0009-9120
VL - 63
SP - 32
EP - 38
JO - Clinical Biochemistry
JF - Clinical Biochemistry
ER -