TY - JOUR
T1 - Cellulose synthesis and cell expansion are regulated by different mechanisms in growing arabidopsis hypocotyls
AU - Ivakov, Alexander
AU - Flis, Anna
AU - Apelt, Federico
AU - Fünfgeld, Maximillian
AU - Scherer, Ulrike
AU - Stitt, Mark
AU - Kragler, Friedrich
AU - Vissenberg, Kris
AU - Persson, Staffan
AU - Suslov, Dmitry
N1 - Funding Information:
A.I. was funded by the Max-Planck Gesellschaft and the University of Melbourne. A.F. was funded by the Max Planck Gesellschaft. S.P. was funded by the Max-Planck Gesellschaft and by a R@MAP professorship at the University of Melbourne and acknowledges funding from an ARC Discovery grant (DP150103495), an ARC Future fellowship grant (FT160100218), and a Hermon-Slade grant (Persson HSF 15/4). D.S. acknowledges Saint Petersburg State University for research grants 1.38.233.2014 and 1.42.1025.2016, the Research Park of Saint Petersburg State University Center for Molecular and Cell Technologies, and RFBR for Grant 15-04-04075. K.V. acknowledges the University of Antwerp and the Research Foundation Flanders (FWO-Vlaanderen) for Grants G.0656.13.N.10, 1.5.091.11.N.00, and G039815N. We acknowledge Ross Dennis for help with imaging.
Publisher Copyright:
© 2017 ASPB.
PY - 2017/6
Y1 - 2017/6
N2 - Plant growth is sustained by two complementary processes: biomass biosynthesis and cell expansion. The cell wall is crucial to both as it forms the majority of biomass, while its extensibility limits cell expansion. Cellulose is a major component of the cell wall and cellulose synthesis is pivotal to plant cell growth, and its regulation is poorly understood. Using periodic diurnal variation in Arabidopsis thaliana hypocotyl growth, we found that cellulose synthesis and cell expansion can be uncoupled and are regulated by different mechanisms. We grew Arabidopsis plants in very short photoperiods and used a combination of extended nights, continuous light, sucrose feeding experiments, and photosynthesis inhibition to tease apart the influences of light, metabolic, and circadian clock signaling on rates of cellulose biosynthesis and cell wall biomechanics. We demonstrate that cell expansion is regulated by protein-mediated changes in cell wall extensibility driven by the circadian clock. By contrast, the biosynthesis of cellulose is controlled through intracellular trafficking of cellulose synthase enzyme complexes regulated exclusively by metabolic signaling related to the carbon status of the plant and independently of the circadian clock or light signaling.
AB - Plant growth is sustained by two complementary processes: biomass biosynthesis and cell expansion. The cell wall is crucial to both as it forms the majority of biomass, while its extensibility limits cell expansion. Cellulose is a major component of the cell wall and cellulose synthesis is pivotal to plant cell growth, and its regulation is poorly understood. Using periodic diurnal variation in Arabidopsis thaliana hypocotyl growth, we found that cellulose synthesis and cell expansion can be uncoupled and are regulated by different mechanisms. We grew Arabidopsis plants in very short photoperiods and used a combination of extended nights, continuous light, sucrose feeding experiments, and photosynthesis inhibition to tease apart the influences of light, metabolic, and circadian clock signaling on rates of cellulose biosynthesis and cell wall biomechanics. We demonstrate that cell expansion is regulated by protein-mediated changes in cell wall extensibility driven by the circadian clock. By contrast, the biosynthesis of cellulose is controlled through intracellular trafficking of cellulose synthase enzyme complexes regulated exclusively by metabolic signaling related to the carbon status of the plant and independently of the circadian clock or light signaling.
UR - http://www.scopus.com/inward/record.url?scp=85025687679&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/28550150
U2 - 10.1105/tpc.16.00782
DO - 10.1105/tpc.16.00782
M3 - Article
C2 - 28550150
AN - SCOPUS:85025687679
SN - 1040-4651
VL - 29
SP - 1305
EP - 1315
JO - Plant Cell
JF - Plant Cell
IS - 6
ER -