TY - JOUR
T1 - Birth mode is associated with earliest strain-conferred gut microbiome functions and immunostimulatory potential
AU - Wampach, Linda
AU - Heintz-Buschart, Anna
AU - Fritz, Joëlle V.
AU - Ramiro-Garcia, Javier
AU - Habier, Janine
AU - Herold, Malte
AU - Narayanasamy, Shaman
AU - Kaysen, Anne
AU - Hogan, Angela H.
AU - Bindl, Lutz
AU - Bottu, Jean
AU - Halder, Rashi
AU - Sjöqvist, Conny
AU - May, Patrick
AU - Andersson, Anders F.
AU - de Beaufort, Carine
AU - Wilmes, Paul
N1 - Funding Information:
In silico analyses presented in this paper were carried out using the HPC facilities of the University of Luxembourg80. We are grateful to all the parents and neonates who participated in the study. We thank the dedicated clinical staff and neonatologists of the paediatric clinic and gynaecologists at the CHL for participant recruitment and sample collection, especially Alain Noirhomme and all involved study nurses of the Clinical and Epidemiological Investigation Center (CIEC) who performed sample and data collection at the CHL and at home as well as the scientific staff of the IBBL for sample storage. We are thankful for the assistance of Audrey Frachet and Janine Habier (LCSB) for laboratory support, Jochen Schneider (LCSB) for discussions, and Lola Kourouma for metadata entry and cross-checking of medical data. Lisa Morgenstern from GATC Biotech AG in Constance is thanked for her work and discussion on random shotgun sequencing and Wouter Coppieters and Latifa Karim from CART-GIGA for their work on 16S rRNA gene amplicon sequencing. We thank the MO BIO technical support team for recommendations on extraction kit optimizations. The present work was partially financed by the Fondation André et Henriette Losch. It was further supported by an ATTRACT programme grant (ATTRACT/A09/03) and CORE programme grants (CORE/15/BM/104040 and CORE/C15/SR/10404839) to P.W. and (CORE Junior/14/ BM/8066232) to J.V.F., Aide à la Formation Recherche grants to L.W. (AFR PHD-2013-5824125) and S.N. (AFR PHD-2014-1/7934898), all funded by the Luxembourg National Research Fund (FNR). A.K. was funded by the University of Luxembourg (ImMicro-Dyn1). Sample collection, processing and storage were co-funded by the Integrated BioBank of Luxembourg under the Personalised Medicine Consortium Diabetes programme.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The rate of caesarean section delivery (CSD) is increasing worldwide. It remains unclear whether disruption of mother-to-neonate transmission of microbiota through CSD occurs and whether it affects human physiology. Here we perform metagenomic analysis of earliest gut microbial community structures and functions. We identify differences in encoded functions between microbiomes of vaginally delivered (VD) and CSD neonates. Several functional pathways are over-represented in VD neonates, including lipopolysaccharide (LPS) biosynthesis. We link these enriched functions to individual-specific strains, which are transmitted from mothers to neonates in case of VD. The stimulation of primary human immune cells with LPS isolated from early stool samples of VD neonates results in higher levels of tumour necrosis factor (TNF-α) and interleukin 18 (IL-18). Accordingly, the observed levels of TNF-α and IL-18 in neonatal blood plasma are higher after VD. Taken together, our results support that CSD disrupts mother-to-neonate transmission of specific microbial strains, linked functional repertoires and immune-stimulatory potential during a critical window for neonatal immune system priming.
AB - The rate of caesarean section delivery (CSD) is increasing worldwide. It remains unclear whether disruption of mother-to-neonate transmission of microbiota through CSD occurs and whether it affects human physiology. Here we perform metagenomic analysis of earliest gut microbial community structures and functions. We identify differences in encoded functions between microbiomes of vaginally delivered (VD) and CSD neonates. Several functional pathways are over-represented in VD neonates, including lipopolysaccharide (LPS) biosynthesis. We link these enriched functions to individual-specific strains, which are transmitted from mothers to neonates in case of VD. The stimulation of primary human immune cells with LPS isolated from early stool samples of VD neonates results in higher levels of tumour necrosis factor (TNF-α) and interleukin 18 (IL-18). Accordingly, the observed levels of TNF-α and IL-18 in neonatal blood plasma are higher after VD. Taken together, our results support that CSD disrupts mother-to-neonate transmission of specific microbial strains, linked functional repertoires and immune-stimulatory potential during a critical window for neonatal immune system priming.
UR - http://www.scopus.com/inward/record.url?scp=85057604219&partnerID=8YFLogxK
U2 - 10.1038/s41467-018-07631-x
DO - 10.1038/s41467-018-07631-x
M3 - Article
C2 - 30504906
AN - SCOPUS:85057604219
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 5091
ER -