TY - JOUR
T1 - Biospecimen Qualification in a Clinical Biobank of Urological Diseases
AU - Kofanova, Olga
AU - Paul, Sangita
AU - Pexaras, Achilleas
AU - Bellora, Camille
AU - Petersons, Ala
AU - Schmitt, Margaux
AU - Baker Berjaoui, Mohamad
AU - Qaoud, Yazan
AU - Kenk, Miran
AU - Wagner, Heidi
AU - Fleshner, Neil
AU - Betsou, Fay
N1 - Publisher Copyright:
© Mary Ann Liebert, Inc.
PY - 2024/6
Y1 - 2024/6
N2 - Development of novel biomarkers for diagnosis of disease and assessment of treatment efficacy utilizes a wide range of biospecimens for discovery research. The fitness of biospecimens for the purpose of biomarker development depends on the clinical characteristics of the donor and on a number of critical and potentially uncontrolled pre-analytical variables. Pre-analytical factors influence the reliability of the biomarkers to be analyzed and can seriously impact analytic outcomes. Sample quality stratification assays and tools can be utilized by biorepositories to minimize bias resulting from samples’ inconsistent quality. In this study, we evaluated the quality of biobanked specimens by comparing analytical outcomes at 1, 5, and 10 years after collection. Our results demonstrate that currently available assays and tools can be used by biobank laboratories to support objective biospecimen qualification. We have established a workflow to monitor the quality of different types of biospecimens and, in this study, present the results of a qualification exercise applied to fluid samples and their derivatives in the context of urological diseases.
AB - Development of novel biomarkers for diagnosis of disease and assessment of treatment efficacy utilizes a wide range of biospecimens for discovery research. The fitness of biospecimens for the purpose of biomarker development depends on the clinical characteristics of the donor and on a number of critical and potentially uncontrolled pre-analytical variables. Pre-analytical factors influence the reliability of the biomarkers to be analyzed and can seriously impact analytic outcomes. Sample quality stratification assays and tools can be utilized by biorepositories to minimize bias resulting from samples’ inconsistent quality. In this study, we evaluated the quality of biobanked specimens by comparing analytical outcomes at 1, 5, and 10 years after collection. Our results demonstrate that currently available assays and tools can be used by biobank laboratories to support objective biospecimen qualification. We have established a workflow to monitor the quality of different types of biospecimens and, in this study, present the results of a qualification exercise applied to fluid samples and their derivatives in the context of urological diseases.
KW - biospecimen
KW - pre-analytical factors
KW - qualification
KW - sample quality stratification
UR - http://www.scopus.com/inward/record.url?scp=85176425999&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/37878356
U2 - 10.1089/bio.2022.0190
DO - 10.1089/bio.2022.0190
M3 - Article
C2 - 37878356
SN - 1947-5535
VL - 22
SP - 257
EP - 267
JO - Biopreservation and Biobanking
JF - Biopreservation and Biobanking
IS - 3
ER -