@article{61f90fe2fc654feaacffde97d9659af1,
title = "Automated high-throughput high-content autophagy and mitophagy analysis platform",
abstract = "Autophagic processes play a central role in cellular homeostasis. In pathological conditions, the flow of autophagy can be affected at multiple and distinct steps of the pathway. Current analyses tools do not deliver the required detail for dissecting pathway intermediates. The development of new tools to analyze autophagic processes qualitatively and quantitatively in a more straightforward manner is required. Defining all autophagy pathway intermediates in a high-throughput manner is technologically challenging and has not been addressed yet. Here, we overcome those requirements and limitations by the developed of stable autophagy and mitophagy reporter-iPSC and the establishment of a novel high-throughput phenotyping platform utilizing automated high-content image analysis to assess autophagy and mitophagy pathway intermediates.",
author = "Jonathan Arias-Fuenzalida and Javier Jarazo and Jonas Walter and Gemma Gomez-Giro and Forster, {Julia I.} and Rejko Krueger and Antony, {Paul M.A.} and Schwamborn, {Jens C.}",
note = "Funding Information: We thank Prof. J. Hejna and E. Berger for critical comments on the manuscript. We would like to thank Prof. T. Graham and A. Sargsyan from the University of Utah for kindly providing us with pHluorin constructs, Prof. R. Jaenisch from the MIT Whitehead Institute for providing the AAVS1 targeting vector, and Prof. F. Zhang from the McGovern Institute for Brain Research for providing the TALEN vectors. This project was supported by the LCSB pluripotent stem cell core facility. This project was funded by the Fonds National de la Recherche (FNR) Luxembourg (CORE, C13/BM/5791363). This is an EU Joint Program - Neurodegenerative Disease Research (JPND) project (INTER/JPND/14/02; INTER/JPND/15/11092422). J.W., J.J. and J.F. were supported by FNR Aides {\`a} la Formation-Recherche (AFR). G.GG. was funded by the NCL-Stiftung (Hamburg, Germany). Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = dec,
day = "1",
doi = "10.1038/s41598-019-45917-2",
language = "English",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}