Autologous cytotoxicity of natural killer cells derived from HIV-infected patients

Anna Linda Hultström; Göran Bratt; Antonio Cosma; Volker Erfle; Britta Wahren; Ennio Carbone

doi:10.1016/j.imlet.2003.11.012

Autologous cytotoxicity of natural killer cells derived from HIV-infected patients

Anna Linda Hultström, Göran Bratt, Antonio Cosma, Volker Erfle, Britta Wahren, Ennio Carbone^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

NK cells recognize target cells with reduced expression of MHC class I molecules. Human immunodeficiency virus (HIV) infection decreases MHC class I on the cell membrane. The aim of this study was to directly evaluate the role and conditions of NK cell effects in HIV seropositive patients ex vivo. Autologous HIV-infected CD4+ T cells were exposed to NK cells recognition. We discovered an increased lysis of the target cells after infection with human immunodeficiency virus-1 (HIV-1). The expression of the HIV-1 nef gene or the combined expression of nef and tat confers NK susceptibility to autologous CD4+ targets. Downregulation of MHC class I but not HLA-C or CD4 correlated with increased recognition by NK cells. The specific recognition is correlated with downregulation of MHC class I molecules on the infected target cells.

Original language	English
Pages (from-to)	155-158
Number of pages	4
Journal	Immunology Letters
Volume	91
Issue number	2-3
DOIs	https://doi.org/10.1016/j.imlet.2003.11.012
Publication status	Published - 15 Feb 2004
Externally published	Yes

Keywords

HIV
MHC class I
NK

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10.1016/j.imlet.2003.11.012

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title = "Autologous cytotoxicity of natural killer cells derived from HIV-infected patients",

abstract = "NK cells recognize target cells with reduced expression of MHC class I molecules. Human immunodeficiency virus (HIV) infection decreases MHC class I on the cell membrane. The aim of this study was to directly evaluate the role and conditions of NK cell effects in HIV seropositive patients ex vivo. Autologous HIV-infected CD4+ T cells were exposed to NK cells recognition. We discovered an increased lysis of the target cells after infection with human immunodeficiency virus-1 (HIV-1). The expression of the HIV-1 nef gene or the combined expression of nef and tat confers NK susceptibility to autologous CD4+ targets. Downregulation of MHC class I but not HLA-C or CD4 correlated with increased recognition by NK cells. The specific recognition is correlated with downregulation of MHC class I molecules on the infected target cells.",

keywords = "HIV, MHC class I, NK",

author = "Hultstr{\"o}m, \{Anna Linda\} and G{\"o}ran Bratt and Antonio Cosma and Volker Erfle and Britta Wahren and Ennio Carbone",

note = "Funding Information: We thank Dr. Quentin Sattentau (NIBSC, Hertfordshire, UK). This work was supported by the Swedish Medical Research Council, EU Demonstration project CT-971265, Cancerfonden and Svenska Sallskapet Lakare Medicinska Forbundet.",

year = "2004",

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day = "15",

doi = "10.1016/j.imlet.2003.11.012",

language = "English",

volume = "91",

pages = "155--158",

journal = "Immunology Letters",

issn = "0165-2478",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Autologous cytotoxicity of natural killer cells derived from HIV-infected patients

AU - Hultström, Anna Linda

AU - Bratt, Göran

AU - Cosma, Antonio

AU - Erfle, Volker

AU - Wahren, Britta

AU - Carbone, Ennio

N1 - Funding Information: We thank Dr. Quentin Sattentau (NIBSC, Hertfordshire, UK). This work was supported by the Swedish Medical Research Council, EU Demonstration project CT-971265, Cancerfonden and Svenska Sallskapet Lakare Medicinska Forbundet.

PY - 2004/2/15

Y1 - 2004/2/15

N2 - NK cells recognize target cells with reduced expression of MHC class I molecules. Human immunodeficiency virus (HIV) infection decreases MHC class I on the cell membrane. The aim of this study was to directly evaluate the role and conditions of NK cell effects in HIV seropositive patients ex vivo. Autologous HIV-infected CD4+ T cells were exposed to NK cells recognition. We discovered an increased lysis of the target cells after infection with human immunodeficiency virus-1 (HIV-1). The expression of the HIV-1 nef gene or the combined expression of nef and tat confers NK susceptibility to autologous CD4+ targets. Downregulation of MHC class I but not HLA-C or CD4 correlated with increased recognition by NK cells. The specific recognition is correlated with downregulation of MHC class I molecules on the infected target cells.

AB - NK cells recognize target cells with reduced expression of MHC class I molecules. Human immunodeficiency virus (HIV) infection decreases MHC class I on the cell membrane. The aim of this study was to directly evaluate the role and conditions of NK cell effects in HIV seropositive patients ex vivo. Autologous HIV-infected CD4+ T cells were exposed to NK cells recognition. We discovered an increased lysis of the target cells after infection with human immunodeficiency virus-1 (HIV-1). The expression of the HIV-1 nef gene or the combined expression of nef and tat confers NK susceptibility to autologous CD4+ targets. Downregulation of MHC class I but not HLA-C or CD4 correlated with increased recognition by NK cells. The specific recognition is correlated with downregulation of MHC class I molecules on the infected target cells.

KW - HIV

KW - MHC class I

KW - NK

UR - https://www.scopus.com/pages/publications/1542302600

U2 - 10.1016/j.imlet.2003.11.012

DO - 10.1016/j.imlet.2003.11.012

M3 - Article

C2 - 15019284

AN - SCOPUS:1542302600

SN - 0165-2478

VL - 91

SP - 155

EP - 158

JO - Immunology Letters

JF - Immunology Letters

IS - 2-3

ER -

Autologous cytotoxicity of natural killer cells derived from HIV-infected patients

Abstract

Keywords

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