TY - JOUR
T1 - Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer
AU - Vladimirov, Sandra
AU - Zeljkovic, Aleksandra
AU - Gojkovic, Tamara
AU - Miljkovic, Milica
AU - Stefanovic, Aleksandra
AU - Zeljkovic, Dejan
AU - Trifunovic, Bratislav
AU - Spasojevic-Kalimanovska, Vesna
N1 - Funding Information:
Funding: This study was financially supported by a grant from the Ministry of Education, Science and Technological Development, Republic of Serbia (Project No. 175035).
Publisher Copyright:
© 2019 Walter de Gruyter GmbH, Berlin/Boston 2019.
PY - 2020/9/2
Y1 - 2020/9/2
N2 - Vitamin D deficiency is repeatedly reported in colorectal cancer (CRC). Since cholesterol and vitamin D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was to analyze relationships between serum 25(OH)D3, 24,25(OH)2D3 and 7-DHC levels and serum lipids in patients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. We recruited 82 patients CRC and 77 controls. 7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods. 7-DHC, 25(OH)D3 and vitamin D metabolic ratio (VDMR) were significantly lower in CRC patients than in control group (P<0.001, P<0.010, P<0.050 and P<0.050, respectively). 25(OH)D3 levels were higher in patients with grade I CRC when compared to grade II (P<0.050). All vitamin D metabolites positively correlated with total cholesterol (TC) concentration in CRC patients. 25(OH)D3 was significant predictor of increased CRC risk (P<0.010). After adjustment for TC concentration, 25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P<0.050). We found significant positive association between vitamin D status and serum total cholesterol. Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status in CRC. However, our results suggest that low 25(OH)D3 might independently contribute to development of poorly differentiated tumor.
AB - Vitamin D deficiency is repeatedly reported in colorectal cancer (CRC). Since cholesterol and vitamin D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was to analyze relationships between serum 25(OH)D3, 24,25(OH)2D3 and 7-DHC levels and serum lipids in patients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. We recruited 82 patients CRC and 77 controls. 7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods. 7-DHC, 25(OH)D3 and vitamin D metabolic ratio (VDMR) were significantly lower in CRC patients than in control group (P<0.001, P<0.010, P<0.050 and P<0.050, respectively). 25(OH)D3 levels were higher in patients with grade I CRC when compared to grade II (P<0.050). All vitamin D metabolites positively correlated with total cholesterol (TC) concentration in CRC patients. 25(OH)D3 was significant predictor of increased CRC risk (P<0.010). After adjustment for TC concentration, 25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P<0.050). We found significant positive association between vitamin D status and serum total cholesterol. Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status in CRC. However, our results suggest that low 25(OH)D3 might independently contribute to development of poorly differentiated tumor.
KW - 24,25-dihydroxyvitamin D
KW - 25-hydroxyvitamin D
KW - 7-dehydrocholesterol
KW - colorectal cancer
KW - total cholesterol
UR - http://www.scopus.com/inward/record.url?scp=85074221486&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/33269020
U2 - 10.2478/jomb-2019-0047
DO - 10.2478/jomb-2019-0047
M3 - Article
C2 - 33269020
AN - SCOPUS:85074221486
SN - 1452-8258
VL - 39
SP - 318
EP - 327
JO - Journal of Medical Biochemistry
JF - Journal of Medical Biochemistry
IS - 3
ER -