Arabidopsis actin-depolymerizing factors (ADFs) 1 and 9 display antagonist activities

Stéphane Tholl, Flora Moreau, Céline Hoffmann, Karthik Arumugam, Monika Dieterle, Danile Moes, Katrin Neumann, André Steinmetz, Clément Thomas*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

We provide evidence that one of the 11 Arabidopsis actin-depolymerizing factors (ADFs), namely ADF9, does not display typical F-actin depolymerizing activity. Instead, ADF9 effectively stabilizes actin filaments in vitro and concomitantly bundles actin filaments with the highest efficiency under acidic conditions. Competition experiments show that ADF9 antagonizes ADF1 activity by reducing its ability to potentiate F-actin depolymerization. Accordingly, ectopic expression of ADF1 and ADF9 in tobacco cells has opposite effects. ADF1 severs actin filaments/bundles and promotes actin cytoskeleton disassembly, whereas ADF9 induces the formation of long bundles. Together these data reveal an additional degree of complexity in the comprehension of the biological functions of the ADF family and illustrate that antagonist activities can be displayed by seemingly equivalent actin-binding proteins.

Original languageEnglish
Pages (from-to)1821-1827
Number of pages7
JournalFEBS Letters
Volume585
Issue number12
DOIs
Publication statusPublished - 23 Jun 2011

Keywords

  • ADF
  • Actin
  • Actin bundling
  • Arabidopsis
  • Cofilin

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