Analysis of the Snake Venom Peptidome

Solange M.T. Serrano; André Zelanis; Jackson G. Miyamoto; Jackelinne Y. Hayashi; Eduardo S. Kitano; Alexandre K. Tashima

doi:10.1007/978-1-0716-3646-6_17

Analysis of the Snake Venom Peptidome

Solange M.T. Serrano, André Zelanis, Jackson G. Miyamoto, Jackelinne Y. Hayashi, Eduardo S. Kitano, Alexandre K. Tashima^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here, we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	319-329
Number of pages	11
Volume	2758
DOIs	https://doi.org/10.1007/978-1-0716-3646-6_17
Publication status	Published - 2024
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2758
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Mass spectrometry
Peptidome
Proteinase inhibition
Snake venom
Solid-phase extraction

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10.1007/978-1-0716-3646-6_17

Cite this

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title = "Analysis of the Snake Venom Peptidome",

abstract = "Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here, we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.",

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AU - Serrano, Solange M.T.

AU - Zelanis, André

AU - Miyamoto, Jackson G.

AU - Hayashi, Jackelinne Y.

AU - Kitano, Eduardo S.

AU - Tashima, Alexandre K.

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

PY - 2024

Y1 - 2024

N2 - Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here, we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.

AB - Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here, we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.

KW - Mass spectrometry

KW - Peptidome

KW - Proteinase inhibition

KW - Snake venom

KW - Solid-phase extraction

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BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Analysis of the Snake Venom Peptidome

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