Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline

Claudia Cerella; Cristina Mearelli; Milena De Nicola; Maria D'Alessio; Andrea Magrini; Antonio Bergamaschi; Lina Ghibelli

doi:10.1196/annals.1387.021

Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline

Claudia Cerella^*, Cristina Mearelli, Milena De Nicola, Maria D'Alessio, Andrea Magrini, Antonio Bergamaschi, Lina Ghibelli

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

6 Citations (Scopus)

Abstract

Many studies suggest that endoplasmic reticulum (ER) Ca²⁺ pool rather than cytosolic Ca²⁺ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca²⁺ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca²⁺ depletor, confirm the findings that apoptotic cells have a Ca²⁺-depleted ER, in contrast with treated but still viable cells.

Original language	English
Title of host publication	Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion
Subtitle of host publication	Fifth International Conference
Publisher	Blackwell Publishing Inc.
Pages	490-493
Number of pages	4
ISBN (Print)	1573316490, 9781573316491
DOIs	https://doi.org/10.1196/annals.1387.021
Publication status	Published - Mar 2007
Externally published	Yes

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1099
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

Apoptosis
Calcium
Chlortetracycline
Endoplasmic reticulum
Thapsigargin

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10.1196/annals.1387.021

Cite this

Cerella, C., Mearelli, C., De Nicola, M., D'Alessio, M., Magrini, A., Bergamaschi, A., & Ghibelli, L. (2007). Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline. In Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference (pp. 490-493). (Annals of the New York Academy of Sciences; Vol. 1099). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1387.021

Cerella, Claudia ; Mearelli, Cristina ; De Nicola, Milena et al. / Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline. Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference. Blackwell Publishing Inc., 2007. pp. 490-493 (Annals of the New York Academy of Sciences).

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title = "Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline",

abstract = "Many studies suggest that endoplasmic reticulum (ER) Ca2+ pool rather than cytosolic Ca2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca2+ depletor, confirm the findings that apoptotic cells have a Ca2+-depleted ER, in contrast with treated but still viable cells.",

keywords = "Apoptosis, Calcium, Chlortetracycline, Endoplasmic reticulum, Thapsigargin",

author = "Claudia Cerella and Cristina Mearelli and {De Nicola}, Milena and Maria D'Alessio and Andrea Magrini and Antonio Bergamaschi and Lina Ghibelli",

year = "2007",

month = mar,

doi = "10.1196/annals.1387.021",

language = "English",

isbn = "1573316490",

series = "Annals of the New York Academy of Sciences",

publisher = "Blackwell Publishing Inc.",

pages = "490--493",

booktitle = "Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion",

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Cerella, C, Mearelli, C, De Nicola, M, D'Alessio, M, Magrini, A, Bergamaschi, A & Ghibelli, L 2007, Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline. in Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference. Annals of the New York Academy of Sciences, vol. 1099, Blackwell Publishing Inc., pp. 490-493. https://doi.org/10.1196/annals.1387.021

Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline. / Cerella, Claudia; Mearelli, Cristina; De Nicola, Milena et al.
Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference. Blackwell Publishing Inc., 2007. p. 490-493 (Annals of the New York Academy of Sciences; Vol. 1099).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline

AU - Cerella, Claudia

AU - Mearelli, Cristina

AU - De Nicola, Milena

AU - D'Alessio, Maria

AU - Magrini, Andrea

AU - Bergamaschi, Antonio

AU - Ghibelli, Lina

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N2 - Many studies suggest that endoplasmic reticulum (ER) Ca2+ pool rather than cytosolic Ca2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca2+ depletor, confirm the findings that apoptotic cells have a Ca2+-depleted ER, in contrast with treated but still viable cells.

AB - Many studies suggest that endoplasmic reticulum (ER) Ca2+ pool rather than cytosolic Ca2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca2+ depletor, confirm the findings that apoptotic cells have a Ca2+-depleted ER, in contrast with treated but still viable cells.

KW - Apoptosis

KW - Calcium

KW - Chlortetracycline

KW - Endoplasmic reticulum

KW - Thapsigargin

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DO - 10.1196/annals.1387.021

M3 - Conference contribution

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AN - SCOPUS:34248512623

SN - 1573316490

SN - 9781573316491

T3 - Annals of the New York Academy of Sciences

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BT - Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion

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Cerella C, Mearelli C, De Nicola M, D'Alessio M, Magrini A, Bergamaschi A et al. Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline. In Sodium-Calcium Exchange and the Plasma Membrane Ca2+ATPase in Ce;; Funcyion: Fifth International Conference. Blackwell Publishing Inc. 2007. p. 490-493. (Annals of the New York Academy of Sciences). doi: 10.1196/annals.1387.021

Analysis of calcium changes in endoplasmic reticulum during apoptosis by the fluorescent indicator chlortetracycline

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