Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS II. LC-MS/MS analysis

Gérard Hopfgartner; Antoine Lesur; Emmanuel Varesio

doi:10.1016/j.trac.2013.03.008

Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS II. LC-MS/MS analysis

Gérard Hopfgartner^*, Antoine Lesur, Emmanuel Varesio

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

36 Citations (Scopus)

Abstract

The first section of Part II describes the current strategies for mass spectrometric (MS) detection in the selected reaction monitoring (SRM) mode which has become the method of choice to quantify peptides. We then discuss the selection of signature peptides, SRM transitions and labeled internal-standard peptides to obtain the best assay selectivity. We also present improved assay selectivity on triple-quadrupole linear ion trap using MS³ and differential mobility MS. We dedicate the final section to alternative approaches based on high-resolution data-independent acquisition.

Original language	English
Pages (from-to)	52-61
Number of pages	10
Journal	TrAC - Trends in Analytical Chemistry
Volume	48
DOIs	https://doi.org/10.1016/j.trac.2013.03.008
Publication status	Published - Jul 2013
Externally published	Yes

Keywords

Biological matrix
Biopharmaceuticals, Peptide
High resolution mass spectrometry, Ion mobility, MSn
Protein, Quantitative analysis, Selected reaction monitoring (SRM), Assay selectivity
Signature peptide

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10.1016/j.trac.2013.03.008

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title = "Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS II. LC-MS/MS analysis",

abstract = "The first section of Part II describes the current strategies for mass spectrometric (MS) detection in the selected reaction monitoring (SRM) mode which has become the method of choice to quantify peptides. We then discuss the selection of signature peptides, SRM transitions and labeled internal-standard peptides to obtain the best assay selectivity. We also present improved assay selectivity on triple-quadrupole linear ion trap using MS3 and differential mobility MS. We dedicate the final section to alternative approaches based on high-resolution data-independent acquisition.",

keywords = "Biological matrix, Biopharmaceuticals, Peptide, High resolution mass spectrometry, Ion mobility, MSn, Protein, Quantitative analysis, Selected reaction monitoring (SRM), Assay selectivity, Signature peptide",

author = "G{\'e}rard Hopfgartner and Antoine Lesur and Emmanuel Varesio",

year = "2013",

month = jul,

doi = "10.1016/j.trac.2013.03.008",

language = "English",

volume = "48",

pages = "52--61",

journal = "TrAC - Trends in Analytical Chemistry",

issn = "0165-9936",

publisher = "Elsevier",

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TY - JOUR

T1 - Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS II. LC-MS/MS analysis

AU - Hopfgartner, Gérard

AU - Lesur, Antoine

AU - Varesio, Emmanuel

PY - 2013/7

Y1 - 2013/7

N2 - The first section of Part II describes the current strategies for mass spectrometric (MS) detection in the selected reaction monitoring (SRM) mode which has become the method of choice to quantify peptides. We then discuss the selection of signature peptides, SRM transitions and labeled internal-standard peptides to obtain the best assay selectivity. We also present improved assay selectivity on triple-quadrupole linear ion trap using MS3 and differential mobility MS. We dedicate the final section to alternative approaches based on high-resolution data-independent acquisition.

AB - The first section of Part II describes the current strategies for mass spectrometric (MS) detection in the selected reaction monitoring (SRM) mode which has become the method of choice to quantify peptides. We then discuss the selection of signature peptides, SRM transitions and labeled internal-standard peptides to obtain the best assay selectivity. We also present improved assay selectivity on triple-quadrupole linear ion trap using MS3 and differential mobility MS. We dedicate the final section to alternative approaches based on high-resolution data-independent acquisition.

KW - Biological matrix

KW - Biopharmaceuticals, Peptide

KW - High resolution mass spectrometry, Ion mobility, MSn

KW - Protein, Quantitative analysis, Selected reaction monitoring (SRM), Assay selectivity

KW - Signature peptide

UR - http://www.scopus.com/inward/record.url?scp=84879462142&partnerID=8YFLogxK

U2 - 10.1016/j.trac.2013.03.008

DO - 10.1016/j.trac.2013.03.008

M3 - Review article

AN - SCOPUS:84879462142

SN - 0165-9936

VL - 48

SP - 52

EP - 61

JO - TrAC - Trends in Analytical Chemistry

JF - TrAC - Trends in Analytical Chemistry

ER -

Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS II. LC-MS/MS analysis

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Keywords

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