Analysis of biopharmaceutical proteins in biological matrices by LC-MS/MS II. LC-MS/MS analysis

Gérard Hopfgartner*, Antoine Lesur, Emmanuel Varesio

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

35 Citations (Scopus)

Abstract

The first section of Part II describes the current strategies for mass spectrometric (MS) detection in the selected reaction monitoring (SRM) mode which has become the method of choice to quantify peptides. We then discuss the selection of signature peptides, SRM transitions and labeled internal-standard peptides to obtain the best assay selectivity. We also present improved assay selectivity on triple-quadrupole linear ion trap using MS3 and differential mobility MS. We dedicate the final section to alternative approaches based on high-resolution data-independent acquisition.

Original languageEnglish
Pages (from-to)52-61
Number of pages10
JournalTrAC - Trends in Analytical Chemistry
Volume48
DOIs
Publication statusPublished - Jul 2013
Externally publishedYes

Keywords

  • Biological matrix
  • Biopharmaceuticals, Peptide
  • High resolution mass spectrometry, Ion mobility, MSn
  • Protein, Quantitative analysis, Selected reaction monitoring (SRM), Assay selectivity
  • Signature peptide

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