TY - JOUR
T1 - An outbreak of African Swine Fever in Nigeria
T2 - Virus isolation and molecular characterization of the VP72 gene of a first isolate from West Africa
AU - Odemuyiwa, Solomon O.
AU - Adebayo, Isaac A.
AU - Ammerlaan, Wim
AU - Ajuwape, Adebowale T.P.
AU - Alaka, Olugbenga O.
AU - Oyedele, Oluwaseyi I.
AU - Soyelu, Kamil O.
AU - Olaleye, David O.
AU - Otesile, Ebenezer B.
AU - Muller, Claude P.
N1 - Funding Information:
We are grateful to Dr. Michael Schreiber of the Department of Virology Bernhard-Nocht Institute of Tropical Medicine, Hamburg, Germany for the synthesis of primers ASF6155 and 6156. Molecular Biology work in the department of Virology, University of Ibadan, was carried out using equipment donated by the Deutscher Akademischer Austausch dienst (DAAD) and the Alexander von Humboldt Stiftung (AvH) to SOO and DOO respectively. SOO was supported by a Ministry of Foreign Affairs fellowship at the Laboratoire National de Santé in Luxembourg.
PY - 2000
Y1 - 2000
N2 - The isolation of 98/ASF/NG, a strain of African Swine Fever Virus (ASFV) associated with a 1998 epizootic in Nigeria, is reported. This first isolate of the virus from West Africa was identified through a successful polymerase chain reaction (PCR) amplification and sequencing of a 280 base pair (bp) fragment of the Major Capsid Protein (VP72) gene. Further amplification and sequence analysis of a 1.9 kilobase pair (kbp) fragment encompassing the complete VP72 gene showed that the isolate has a 92.2%, 92.4%, and 97.2% homology with previously sequenced Ugandan, Dominican Republican and Spanish isolates respectively. Of the 50 nucleotide changes observed in this highly conserved gene, 45 were found to result in 40 amino acid changes clustered around the central region (position 426 to 516) of the VP72 protein while changes at the remaining 5 positions were silent. These changes also led to the loss of two out of the seven potential N-glycosylation sites which are in this gene conserved among all isolates. The possible epizootiological implications of such mutations in a highly conserved gene of a DNA virus is discussed in relation to this outbreak.
AB - The isolation of 98/ASF/NG, a strain of African Swine Fever Virus (ASFV) associated with a 1998 epizootic in Nigeria, is reported. This first isolate of the virus from West Africa was identified through a successful polymerase chain reaction (PCR) amplification and sequencing of a 280 base pair (bp) fragment of the Major Capsid Protein (VP72) gene. Further amplification and sequence analysis of a 1.9 kilobase pair (kbp) fragment encompassing the complete VP72 gene showed that the isolate has a 92.2%, 92.4%, and 97.2% homology with previously sequenced Ugandan, Dominican Republican and Spanish isolates respectively. Of the 50 nucleotide changes observed in this highly conserved gene, 45 were found to result in 40 amino acid changes clustered around the central region (position 426 to 516) of the VP72 protein while changes at the remaining 5 positions were silent. These changes also led to the loss of two out of the seven potential N-glycosylation sites which are in this gene conserved among all isolates. The possible epizootiological implications of such mutations in a highly conserved gene of a DNA virus is discussed in relation to this outbreak.
KW - 98/ASF/NG
KW - ASE
KW - Glycosylation site loss
KW - Mutations
KW - Nigeria
KW - VP72 gene
UR - http://www.scopus.com/inward/record.url?scp=0034119425&partnerID=8YFLogxK
U2 - 10.1023/A:1008118531316
DO - 10.1023/A:1008118531316
M3 - Article
C2 - 10872875
AN - SCOPUS:0034119425
SN - 0920-8569
VL - 20
SP - 139
EP - 142
JO - Virus Genes
JF - Virus Genes
IS - 2
ER -