An antigen microarray protocol for COVID-19 serological analysis

Joseph Longworth; Gunnar Dittmar

doi:10.1016/j.xpro.2021.100815

An antigen microarray protocol for COVID-19 serological analysis

Joseph Longworth
, Gunnar Dittmar^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.

Original language	English
Article number	100815
Pages (from-to)	100815
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100815
Publication status	Published - 17 Sept 2021

Keywords

Antibody
Bioinformatics
Health Sciences
High Throughput Screening
Immunology
Proteomics

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10.1016/j.xpro.2021.100815Licence: CC BY

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title = "An antigen microarray protocol for COVID-19 serological analysis",

abstract = "The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.",

keywords = "Antibody, Bioinformatics, Health Sciences, High Throughput Screening, Immunology, Proteomics",

author = "Joseph Longworth and Gunnar Dittmar",

note = "Funding Information: The COV-Array project is supported by the Luxembourg National Research Fund (FNR) (COV-Array, grant number 14879195 ) of the FNR (Luxembourg). We are thankful to the study participants, the Predi-COVID study group, the national ethics committee, and the funders for their support in the set-up of this initiative. The Predi-COVID study is supported by the Luxembourg National Research Fund (FNR) (Predi-COVID, grant number 14716273 ) and the Andr{\'e} Losch Fondation . We thank Professor David C. James and Dr. Stephen R. P. Jaff{\'e} for kind provision of S1 protein produced in CHO cells. We would like to thank Dr. Christiane Hilger for discussion. We thank Dr. Daniel Perez Hernandez and Dr. Marta Mendes for testing the R application and providing feedback. Publisher Copyright: {\textcopyright} 2021 The Author(s)",

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doi = "10.1016/j.xpro.2021.100815",

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AU - Dittmar, Gunnar

N1 - Funding Information: The COV-Array project is supported by the Luxembourg National Research Fund (FNR) (COV-Array, grant number 14879195 ) of the FNR (Luxembourg). We are thankful to the study participants, the Predi-COVID study group, the national ethics committee, and the funders for their support in the set-up of this initiative. The Predi-COVID study is supported by the Luxembourg National Research Fund (FNR) (Predi-COVID, grant number 14716273 ) and the André Losch Fondation . We thank Professor David C. James and Dr. Stephen R. P. Jaffé for kind provision of S1 protein produced in CHO cells. We would like to thank Dr. Christiane Hilger for discussion. We thank Dr. Daniel Perez Hernandez and Dr. Marta Mendes for testing the R application and providing feedback. Publisher Copyright: © 2021 The Author(s)

PY - 2021/9/17

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N2 - The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.

AB - The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.

KW - Antibody

KW - Bioinformatics

KW - Health Sciences

KW - High Throughput Screening

KW - Immunology

KW - Proteomics

UR - https://www.scopus.com/pages/publications/85122829175

UR - https://pubmed.ncbi.nlm.nih.gov/34462737

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DO - 10.1016/j.xpro.2021.100815

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SP - 100815

JO - STAR Protocols

JF - STAR Protocols

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ER -

An antigen microarray protocol for COVID-19 serological analysis

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Keywords

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