An antigen microarray protocol for COVID-19 serological analysis

Joseph Longworth, Gunnar Dittmar*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)


The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.

Original languageEnglish
Article number100815
Pages (from-to)100815
JournalSTAR Protocols
Issue number3
Publication statusPublished - 17 Sept 2021


  • Antibody
  • Bioinformatics
  • Health Sciences
  • High Throughput Screening
  • Immunology
  • Proteomics


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