Abstract
Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.
Original language | English |
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Pages (from-to) | 3-6 |
Number of pages | 4 |
Journal | BioTechniques |
Volume | 44 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jun 2008 |
Externally published | Yes |