TY - JOUR
T1 - Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy
AU - Grosch, Johannes
AU - Lesur, Antoine
AU - Kler, Stéphanie
AU - Bernardin, François
AU - Dittmar, Gunnar
AU - Francescato, Elisabetta
AU - Hewings, Simon J.
AU - Jakwerth, Constanze A.
AU - Zissler, Ulrich M.
AU - Heath, Matthew D.
AU - Ollert, Markus
AU - Kramer, Matthias F.
AU - Hilger, Christiane
AU - Bilò, Maria Beatrice
AU - Schmidt-Weber, Carsten B.
AU - Blank, Simon
N1 - Funding: This study was financed in part by Bencard Allergie GmbH/Allergy Therapeutics PLC, which is the manufacturer of one of the analyzed products. The company did not influence the study design; the collection, analysis, and interpretation of data; or the decision to submit the article for publication. S.J.H., M.D.H., and M.F.K., who are employees of Bencard Allergie GmbH/Allergy Therapeutics PLC, critically revised the final version of the manuscript but did not influence the interpretation or representation of data. Moreover, this work was supported by the Helmholtz Association, Future Topic “Immunology and Inflammation” (ZT-0027) to S.B. and C.B.S.-W.
PY - 2022/4
Y1 - 2022/4
N2 - Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of P. dominula venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with P. dominula venom and one product with a venom mixture of American Polistes species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American Polistes species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated P. dominula allergens were detected at comparable levels in LC-MS/MS analysis of products containing P. dominula venom. Due to a lack of genomic information on the American Polistes species, the remaining products were not analyzed by this method. The major Polistes allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of P. dominula venom allergy.
AB - Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of P. dominula venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with P. dominula venom and one product with a venom mixture of American Polistes species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American Polistes species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated P. dominula allergens were detected at comparable levels in LC-MS/MS analysis of products containing P. dominula venom. Due to a lack of genomic information on the American Polistes species, the remaining products were not analyzed by this method. The major Polistes allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of P. dominula venom allergy.
KW - allergen-specific immunotherapy
KW - American Polistes species
KW - European paper wasp
KW - European Polistes species
KW - Hymenoptera venom allergy
KW - Polistes dominula
KW - therapeutic venom extract
KW - venom immunotherapy
UR - http://www.scopus.com/inward/record.url?scp=85128681698&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/35448893
U2 - 10.3390/toxins14040284
DO - 10.3390/toxins14040284
M3 - Article
C2 - 35448893
AN - SCOPUS:85128681698
SN - 2072-6651
VL - 14
JO - Toxins
JF - Toxins
IS - 4
M1 - 284
ER -