TY - UNPB
T1 - Akkermansia muciniphila regulates food allergy in a diet-dependent manner
AU - Parrish, Amy
AU - Boudaud, Marie
AU - Grant, Erica
AU - Willieme, Stephanie
AU - Neumann, Mareike
AU - Wolter, Mathis
AU - Craig, Sophie
AU - De Sciscio, Alessandro
AU - Cosma, Antonio
AU - Hunewald, Oliver
AU - Ollert, Markus
AU - Desai, Mahesh
N1 - Acknowledgements:
M.S.D. was supported by the Luxembourg National Research Fund (FNR) CORE grants (C15/BM/10318186 and C18/BM/12585940); A.P. was supported by an FNR AFR individual PhD fellowship (11602973); E.T.G. was supported by FNR PRIDE (17/11823097) and the Fondation du Pélican de Mie et Pierre Hippert-Faber, under the aegis of the Fondation de Luxembourg; M.B. was supported by a European Commission Horizon 2020 Marie Skłodowska-Curie Actions individual fellowship (897408); S.Z.C. was supported by FNR PRIDE (19/14254520); and M.N. was supported by the FNR AFR bilateral grant (15/11228353). We acknowledge the National Cytometry Platform (NCP) and the Cytométrie Pitié- Salpêtrière (CyPS) for their assistance with generating cytometry data. The NCP is supported by Luxembourg's Ministry of Higher Education and Research (MESR) funding. We also gratefully acknowledge the support of Christian Jäger, Xiangyi Dong and Floriane Gavotto from the Luxembourg Centre for Systems Biomedicine (LCSB) Metabolomics Platform for GC-MS analyses.
PY - 2022/7/13
Y1 - 2022/7/13
N2 - Alterations in the gut microbiome, including diet-driven changes, are linked to the rising prevalence of food allergy, yet little is known about how specific gut bacteria incite breakdown of oral tolerance. Here, we show that depriving specific-pathogen-free mice of dietary fiber leads to an increase of the mucolytic bacterium Akkermansia muciniphila, which is associated with a surge in the colonic type 2 immune cells and IgE-coated commensals, and microbiota-mediated gut mucosal barrier dysfunction. These changes manifest into exacerbated sensitization to food allergens, ovalbumin and peanut. To demonstrate the causal role of A. muciniphila, we employed a tractable synthetic human gut microbiota in gnotobiotic mice. The presence of A. muciniphila within the microbiota, combined with fiber deprivation, resulted in stronger anti-commensal IgE coating and type 2 immune responses, which worsened symptoms of food allergy. Our study supports a mechanistic link between diet and a mucolytic gut microbe in regulating food allergy.
AB - Alterations in the gut microbiome, including diet-driven changes, are linked to the rising prevalence of food allergy, yet little is known about how specific gut bacteria incite breakdown of oral tolerance. Here, we show that depriving specific-pathogen-free mice of dietary fiber leads to an increase of the mucolytic bacterium Akkermansia muciniphila, which is associated with a surge in the colonic type 2 immune cells and IgE-coated commensals, and microbiota-mediated gut mucosal barrier dysfunction. These changes manifest into exacerbated sensitization to food allergens, ovalbumin and peanut. To demonstrate the causal role of A. muciniphila, we employed a tractable synthetic human gut microbiota in gnotobiotic mice. The presence of A. muciniphila within the microbiota, combined with fiber deprivation, resulted in stronger anti-commensal IgE coating and type 2 immune responses, which worsened symptoms of food allergy. Our study supports a mechanistic link between diet and a mucolytic gut microbe in regulating food allergy.
U2 - 10.21203/rs.3.rs-1745691/v1
DO - 10.21203/rs.3.rs-1745691/v1
M3 - Preprint
BT - Akkermansia muciniphila regulates food allergy in a diet-dependent manner
ER -