Advances in high-resolution quantitative proteomics: Implications for clinical applications

Sebastien Gallien, Bruno Domon*

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    27 Citations (Scopus)

    Abstract

    The advances in high-resolution mass spectrometry instrumentation, capable of accurate mass measurement and fast acquisition, have enabled new approaches for targeted quantitative proteomics. More specifically, analyses performed on quadrupole-orbitrap mass spectrometers operated in parallel reaction monitoring (PRM) mode leverage the intrinsic high resolving power and trapping capabilities. The PRM technique offers unmatched degrees of selectivity and analytical sensitivity, typically required to analyze peptides in complex samples, such as those encountered in biomedical research or clinical studies. The features of PRM have provoked a paradigm change in targeted experiments, by decoupling acquisition and data processing. It has resulted in a new analytical workflow comprising distinct methods for each step, thus enabling much larger flexibility. The PRM technique was further enhanced by a new data acquisition scheme, allowing dynamic parameter settings. The potential of the technique may radically impact future quantitative proteomics studies.

    Original languageEnglish
    Pages (from-to)489-498
    Number of pages10
    JournalExpert Review of Proteomics
    Volume12
    Issue number5
    DOIs
    Publication statusPublished - 3 Sep 2015

    Keywords

    • high-resolution and accurate-mass
    • multiple reaction monitoring
    • parallel reaction monitoring
    • quadrupole-orbitrap
    • selected reaction monitoring
    • targeted proteomics

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