A quality control of proteomic experiments based on multiple isotopologous internal standards

Adele Bourmaud, Sebastien Gallien, Bruno Domon*

*Corresponding author for this work

    Research output: Contribution to journalArticleResearchpeer-review

    9 Citations (Scopus)

    Abstract

    The harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and perform a system suitability test. The method relies on a straightforward protocol using a simple mixture of exogenous proteins, and the sequential addition of two sets of isotopically labeled peptides added to reference samples. This internal quality control procedure was applied to plasma samples to demonstrate its easy implementation, which makes it generic for most proteomics applications.

    Original languageEnglish
    Pages (from-to)16-21
    Number of pages6
    JournalEuPA Open Proteomics
    Volume8
    DOIs
    Publication statusPublished - 1 Sep 2015

    Keywords

    • Internal standard
    • Isotopically labeled peptides
    • PRM
    • Quality control
    • SRM

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