TY - JOUR
T1 - A new recombinant virus system for the study of HIV-1 entry and inhibition
AU - Roman, Francois
AU - Ammerlaan, Wim
AU - Plesseria, Jean Marc
AU - Deroo, Sabrina
AU - Muller, Claude P.
AU - Arendt, Vic
AU - Schneider, Francois
AU - Hemmer, Robert
AU - Schmit, Jean Claude
N1 - Funding Information:
This work was supported by the ‘Fondation Recherche sur le SIDA’ and the ‘Centre de Recherche Publique-Santé’ (CRP-Santé), Luxembourg, and in part by a GlaxoSmithKline grant awarded by the Belgian society for the Study of Infectiology and Clinical Microbiology (SBIMC-BVIKM). F.R. acknowledges a grant (BFR99/003) from the ‘Ministère de la Culture, de l’Enseignement Supérieur et de la Recherche’, Luxembourg (1999–2002). The following reagents were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: pNL4-3 from Dr. Malcolm Martin and HXB2-env from Dr. Kathleen Page and Dr. Dan Littman.
PY - 2006/1
Y1 - 2006/1
N2 - The construction is described of a HIV-1 proviral, eGFP-tagged plasmid that allows for the recombination of any selected env gene without the use of restriction enzymes and for the quantitation of the infection by the recombinant virus using flow cytometry. The system was tested showing that an isoleucine to valine substitution at residue position 37 of the HIV-1 gp41 impairs the fitness of the virus but does not lead by itself to T-20 resistance.
AB - The construction is described of a HIV-1 proviral, eGFP-tagged plasmid that allows for the recombination of any selected env gene without the use of restriction enzymes and for the quantitation of the infection by the recombinant virus using flow cytometry. The system was tested showing that an isoleucine to valine substitution at residue position 37 of the HIV-1 gp41 impairs the fitness of the virus but does not lead by itself to T-20 resistance.
UR - http://www.scopus.com/inward/record.url?scp=28844489915&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/16111774
U2 - 10.1016/j.jviromet.2005.07.005
DO - 10.1016/j.jviromet.2005.07.005
M3 - Article
C2 - 16111774
AN - SCOPUS:28844489915
SN - 0166-0934
VL - 131
SP - 99
EP - 104
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -