@article{1aed5393535c4df0a619e3cf8b46a689,
title = "A new recombinant virus system for the study of HIV-1 entry and inhibition",
abstract = "The construction is described of a HIV-1 proviral, eGFP-tagged plasmid that allows for the recombination of any selected env gene without the use of restriction enzymes and for the quantitation of the infection by the recombinant virus using flow cytometry. The system was tested showing that an isoleucine to valine substitution at residue position 37 of the HIV-1 gp41 impairs the fitness of the virus but does not lead by itself to T-20 resistance.",
author = "Francois Roman and Wim Ammerlaan and Plesseria, {Jean Marc} and Sabrina Deroo and Muller, {Claude P.} and Vic Arendt and Francois Schneider and Robert Hemmer and Schmit, {Jean Claude}",
note = "Funding Information: This work was supported by the {\textquoteleft}Fondation Recherche sur le SIDA{\textquoteright} and the {\textquoteleft}Centre de Recherche Publique-Sant{\'e}{\textquoteright} (CRP-Sant{\'e}), Luxembourg, and in part by a GlaxoSmithKline grant awarded by the Belgian society for the Study of Infectiology and Clinical Microbiology (SBIMC-BVIKM). F.R. acknowledges a grant (BFR99/003) from the {\textquoteleft}Minist{\`e}re de la Culture, de l{\textquoteright}Enseignement Sup{\'e}rieur et de la Recherche{\textquoteright}, Luxembourg (1999–2002). The following reagents were obtained through the AIDS Research and Reference Reagent Program, Division of AIDS, NIAID, NIH: pNL4-3 from Dr. Malcolm Martin and HXB2-env from Dr. Kathleen Page and Dr. Dan Littman.",
year = "2006",
month = jan,
doi = "10.1016/j.jviromet.2005.07.005",
language = "English",
volume = "131",
pages = "99--104",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier B.V.",
number = "1",
}