A multiplex TaqMan PCR assay for the detection of measles and rubella virus

Judith M. Hübschen; Jacques R. Kremer; Sébastien De Landtsheer; Claude P. Muller

doi:10.1016/j.jviromet.2008.01.032

A multiplex TaqMan PCR assay for the detection of measles and rubella virus

Judith M. Hübschen
, Jacques R. Kremer
, Sébastien De Landtsheer
, Claude P. Muller^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

83 Citations (Scopus)

Abstract

Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.

Original language	English
Pages (from-to)	246-250
Number of pages	5
Journal	Journal of Virological Methods
Volume	149
Issue number	2
DOIs	https://doi.org/10.1016/j.jviromet.2008.01.032
Publication status	Published - May 2008

Keywords

Detection
Measles
Multiplex
PCR
Real-time
Rubella

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10.1016/j.jviromet.2008.01.032

Cite this

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title = "A multiplex TaqMan PCR assay for the detection of measles and rubella virus",

abstract = "Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.",

keywords = "Detection, Measles, Multiplex, PCR, Real-time, Rubella",

author = "H{\"u}bschen, \{Judith M.\} and Kremer, \{Jacques R.\} and \{De Landtsheer\}, S{\'e}bastien and Muller, \{Claude P.\}",

note = "Funding Information: This work was supported by the Ministry of Foreign Affairs and the Centre de Recherche Public-Sant{\'e}, Luxembourg. We thank Dr. Joseph P. Icenogle and Emily S. Abernathy from the Centers for Disease Control and Prevention, Atlanta, USA, Dr. Sabine Santibanez from the Robert Koch Institute Berlin, Germany and Dr. Fran{\c c}ois Schneider from the Laboratoire National de Sant{\'e}, Luxembourg, for providing virus strains. ",

year = "2008",

month = may,

doi = "10.1016/j.jviromet.2008.01.032",

language = "English",

volume = "149",

pages = "246--250",

journal = "Journal of Virological Methods",

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publisher = "Elsevier B.V.",

number = "2",

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TY - JOUR

T1 - A multiplex TaqMan PCR assay for the detection of measles and rubella virus

AU - Hübschen, Judith M.

AU - Kremer, Jacques R.

AU - De Landtsheer, Sébastien

AU - Muller, Claude P.

N1 - Funding Information: This work was supported by the Ministry of Foreign Affairs and the Centre de Recherche Public-Santé, Luxembourg. We thank Dr. Joseph P. Icenogle and Emily S. Abernathy from the Centers for Disease Control and Prevention, Atlanta, USA, Dr. Sabine Santibanez from the Robert Koch Institute Berlin, Germany and Dr. François Schneider from the Laboratoire National de Santé, Luxembourg, for providing virus strains.

PY - 2008/5

Y1 - 2008/5

N2 - Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.

AB - Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.

KW - Detection

KW - Measles

KW - Multiplex

KW - PCR

KW - Real-time

KW - Rubella

UR - https://www.scopus.com/pages/publications/41349091208

UR - https://pubmed.ncbi.nlm.nih.gov/18353451

U2 - 10.1016/j.jviromet.2008.01.032

DO - 10.1016/j.jviromet.2008.01.032

M3 - Article

C2 - 18353451

AN - SCOPUS:41349091208

SN - 0166-0934

VL - 149

SP - 246

EP - 250

JO - Journal of Virological Methods

JF - Journal of Virological Methods

IS - 2

ER -

A multiplex TaqMan PCR assay for the detection of measles and rubella virus

Abstract

Keywords

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