A multiplex TaqMan PCR assay for the detection of measles and rubella virus

Judith M. Hübschen, Jacques R. Kremer, Sébastien De Landtsheer, Claude P. Muller*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

63 Citations (Scopus)

Abstract

Measles and rubella virus cause fever/rash diseases that are difficult to differentiate clinically. Both viruses can be detected in the same clinical specimens and are propagated on the same cell cultures. A single-tube multiplex TaqMan assay is described for the simultaneous and rapid detection of the full spectrum of known genetic variants. The performance of the assay is similar to a conventional nested PCR and generates cDNA with random primers which can be used directly for virus genotyping.

Original languageEnglish
Pages (from-to)246-250
Number of pages5
JournalJournal of Virological Methods
Volume149
Issue number2
DOIs
Publication statusPublished - May 2008

Keywords

  • Detection
  • Measles
  • Multiplex
  • PCR
  • Real-time
  • Rubella

Fingerprint

Dive into the research topics of 'A multiplex TaqMan PCR assay for the detection of measles and rubella virus'. Together they form a unique fingerprint.

Cite this