@article{f14c01e6489d4a16a9cee85c1cb64204,
title = "A Dietary Fiber-Deprived Gut Microbiota Degrades the Colonic Mucus Barrier and Enhances Pathogen Susceptibility",
abstract = "Despite the accepted health benefits of consuming dietary fiber, little is known about the mechanisms by which fiber deprivation impacts the gut microbiota and alters disease risk. Using a gnotobiotic mouse model, in which animals were colonized with a synthetic human gut microbiota composed of fully sequenced commensal bacteria, we elucidated the functional interactions between dietary fiber, the gut microbiota, and the colonic mucus barrier, which serves as a primary defense against enteric pathogens. We show that during chronic or intermittent dietary fiber deficiency, the gut microbiota resorts to host-secreted mucus glycoproteins as a nutrient source, leading to erosion of the colonic mucus barrier. Dietary fiber deprivation, together with a fiber-deprived, mucus-eroding microbiota, promotes greater epithelial access and lethal colitis by the mucosal pathogen, Citrobacter rodentium. Our work reveals intricate pathways linking diet, the gut microbiome, and intestinal barrier dysfunction, which could be exploited to improve health using dietary therapeutics.",
keywords = "Akkermansia, Citrobacter rodentium, bacteroides, dietary fiber, gylcans, microbiome, microbiota, mucin, mucus layer, polysaccharides",
author = "Desai, {Mahesh S.} and Seekatz, {Anna M.} and Koropatkin, {Nicole M.} and Nobuhiko Kamada and Hickey, {Christina A.} and Mathis Wolter and Pudlo, {Nicholas A.} and Sho Kitamoto and Nicolas Terrapon and Arnaud Muller and Young, {Vincent B.} and Bernard Henrissat and Paul Wilmes and Stappenbeck, {Thaddeus S.} and Gabriel N{\'u}{\~n}ez and Martens, {Eric C.}",
note = "Funding Information: We thank Lansing C. Hoskins for critical comments on this manuscript and the germfree animal facility of the University of Michigan for expert support. We also thank Markus Ollert and Rudi Balling for their encouragement and advice. This work was supported by Luxembourg National Research Fund (FNR) INTER Mobility ( 13/5624108 ) and CORE ( C15/BM/10318186 ) grants to M.S.D.; Luxembourg Ministry of Higher Education and Research support (DM-Muc) to M.S.D.; FNR ATTRACT ( A09/03 ), CORE ( 11/1186762 ), and European Union Joint Programming in Neurodegenerative Diseases ( INTER/JPND/12/01 ) grants to P.W.; NIH R01 ( GM099513 ) grant to E.C.M.; and financial support from the University of Michigan Host Microbiome Initiative and Center for Gastrointestinal Research ( DK034933 ). Publisher Copyright: {\textcopyright} 2016 Elsevier Inc.",
year = "2016",
month = nov,
day = "17",
doi = "10.1016/j.cell.2016.10.043",
language = "English",
volume = "167",
pages = "1339--1353.e21",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "5",
}