A cerebral glioma model for experimental therapy and in vivo invasion studies in syngeneic BD IX rats

Olav Mella*, Rolf Bjerkvig, Baard Christian Schem, Olav Dahl, Ole Didrik Laerum

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

28 Citations (Scopus)


An in vivo glioma model was developed in syngeneic BD IX rats. The BT4An tumor was derived from serial in vivo passages of the BT4A tumor, originally induced from transformed fetal rat brain cells after transplacental exposure to ethylnitrosourea. The cell line was characterized for the presence of neuroglial differentiation markers, chromosome content and cell cycle distribution as determined by flowcytometry. A standardized method for i.c. tumor induction was developed, and the tumors were investigated by light and electron microscopy and for evidence of blood-brain barrier disruption. Tumor cell ability for phagocytosis was studied, as this property may be important for the invasion pattern of the tumors. We conclude that the model seems suitable for both in vivo therapy and invasion studies. The tumor had 100% tumor take, yielded a predictable symptom-free life span after inoculation, had a characteristic histological picture of an aggressive glioma, and the blood-brain barrier within the tumor was in part disrupted. Compared to the parent cell line, there was loss of neuroglial differentiation markers, the chromosomal distribution was changed, and the ability for phagocytosis was practically lost.

Original languageEnglish
Pages (from-to)93-104
Number of pages12
JournalJournal of Neuro-Oncology
Issue number2
Publication statusPublished - Oct 1990
Externally publishedYes


  • blood-brain barrier
  • differentiation markers
  • experimental brain tumor
  • glioma


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