2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain

Matias Pasquali; Tommaso Serchi; Jenny Renaut; Lucien Hoffmann; Torsten Bohn

doi:10.1002/elps.201200256

2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain

Matias Pasquali, Tommaso Serchi^*, Jenny Renaut, Lucien Hoffmann, Torsten Bohn

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol-producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI-TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.

Original language	English
Pages (from-to)	505-509
Number of pages	5
Journal	Electrophoresis
Volume	34
Issue number	4
DOIs	https://doi.org/10.1002/elps.201200256
Publication status	Published - Feb 2013
Externally published	Yes

Keywords

Fungal proteomics
Genome annotation
Proteome reference map
Trichothecene

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10.1002/elps.201200256

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title = "2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain",

abstract = "Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol-producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI-TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.",

keywords = "Fungal proteomics, Genome annotation, Proteome reference map, Trichothecene",

author = "Matias Pasquali and Tommaso Serchi and Jenny Renaut and Lucien Hoffmann and Torsten Bohn",

year = "2013",

month = feb,

doi = "10.1002/elps.201200256",

language = "English",

volume = "34",

pages = "505--509",

journal = "Electrophoresis",

issn = "0173-0835",

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TY - JOUR

T1 - 2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain

AU - Pasquali, Matias

AU - Serchi, Tommaso

AU - Renaut, Jenny

AU - Hoffmann, Lucien

AU - Bohn, Torsten

PY - 2013/2

Y1 - 2013/2

N2 - Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol-producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI-TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.

AB - Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2D DIGE reference map for the nivalenol-producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI-TOF/TOF, leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F. graminearum biology and chemotype diversity.

KW - Fungal proteomics

KW - Genome annotation

KW - Proteome reference map

KW - Trichothecene

UR - https://www.scopus.com/pages/publications/84873988384

U2 - 10.1002/elps.201200256

DO - 10.1002/elps.201200256

M3 - Article

C2 - 23172383

AN - SCOPUS:84873988384

SN - 0173-0835

VL - 34

SP - 505

EP - 509

JO - Electrophoresis

JF - Electrophoresis

IS - 4

ER -

2D difference gel electrophoresis reference map of a Fusarium graminearum nivalenol producing strain

Abstract

Keywords

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