ПЕРСПЕКТИВНЫЙ МЕТОД ОЧИСТКИ ОБРАЗЦОВ ДЛЯ ИССЛЕДОВАНИЙ ВИРОМОВ

Translated title of the contribution: Perspective method of samples purification for viromes research

E.O. Rubalskii*, N.T. Tikhonova, V.A. Aleshkin, Claude P. Muller, Judith M. Hübschen, M.G. Chikobava

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

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Abstract

Metagenomic researches of viral communities (viromes) are currently one of the most dynamically developing approaches for the detection of rare, mutant and discoveries of previously unknown viruses. High-throughput sequencing methods are widely used in such researches. An important factor limiting metagenomic researches of viruses is a presence in analyzed samples cell-free eukaryotic and prokaryotic nucleic acids (NA), the size of which is much more than viral NA. The known methods for non-viral NA depletion or viral particles enrichment (concentration) require a relatively large amount of input material, are insufficiently universal and not suitable for samples containing partially degraded virions. Our method does not have these disadvantages and allows the universal purification from free NA for samples potentially containing viral particles at least 4 times.
Translated title of the contributionPerspective method of samples purification for viromes research
Original languageRussian
Pages (from-to)19-24
JournalKursk Scientific and Practical Bulletin "Man and his Health"
Volume4
Publication statusPublished - 2014

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